The elastic properties of single double-stranded DNA chains of different lengths as measured with optical tweezers

نویسندگان

  • M. Salomo
  • K. Kegler
  • C. Gutsche
  • M. Struhalla
  • J. Reinmuth
  • W. Skokow
  • U. Hahn
  • F. Kremer
چکیده

Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: ∼2.0 μm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10-12 N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force-extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found.

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عنوان ژورنال:

دوره 284  شماره 

صفحات  -

تاریخ انتشار 2006